Comparison of environmental DNA sampling protocols for detection of stream fishes in the Pacific Northwest, 2019

This dataset contains environmental DNA (eDNA) data (field, extraction, and qPCR) for rainbow trout sampling efforts conducted in the Pacific Northwest in 2019. Samples were collected using three eDNA sampling methods. Also included are qPCR data for extraction negatives, no-template controls (NTCs), and standards (serial dilutions of rainbow trout gBlock). Data for one environmental covariate not collected in the field (stream discharge) were obtained from external sources (USGS 2024, WSC 2024).