Cytokine levels in serum of Ifnar−/− and CD11c Cre+Ifnarf/f mice after treatment with anti-TNF-α MAbs and WNV infection.

CD11c Cre+Ifnarf/f and Ifnar−/− mice were treated with either anti-TNF-α blocking or isotype MAb (200 µg/mouse) one day prior to infection (n = 8 for each group). The mice were infected with 102 PFU of WNV and at 48 hours serum was collected and the concentration of cytokines was determined. Mean values ± SD are shown. P values are compared to the isotype control MAb treated samples for each group. Data are pooled from three independent experiments. ns indicates results that are not statistically different compared to mice treated with isotype control MAb. Not detected (ND) indicates samples that were below the limit of detection for the assay.