Freshwater Reservoir Tous Metagenome Genome sequencing and assembly. freshwater metagenome

The study aims to describe the microbial community of a freshwater reservoir using high throughput sequencing. The reservoir is called Tous and is located in the region of Valencia, Spain. Water was collected and filtered through a series of polycarbonate filters, a 20 micron prefilter, 5 micron (for large cells and eukaryotes) and 0.22 (for planktonic microbes). DNA was extracted from the 0.22 micron filter (small, planktonic fraction) filter to obtain genomic DNA of the microbial community. The samples were sequenced by a shotgun metagenomic approach. All sequencing was done using Illumina HiSeq. Metagenomic reads are available within this bioproject.