Histone citrullination in estrogen receptor signaling and breast cancer [ChIP-seq]

PAD2, one of a family of PAD enzymes that convert positively charged arginine residues on target proteins to neutrally charged citrulline, has been shown to play a role in estrogen signaling through interactions with histone H3R26. The objectives of this project are to define the role of PAD2 in estrogen receptor alpha signaling, tumor progression, and tamoxifen resistance. Overall design: ChIP-seq was utilized to investigate the interaction between citrullination and PAD2 upon estrogen treatment in MCF7 cells.