Aerobicity stimulon in Escherichia coli revealed using multi-scale computational systems biology of adapted respiratory variants

Respiratory variants of E. coli were evolved in succinate and glycerol as carbon sources, to understand their metabolic flexibility and rewiring to attain energy homeostatis. Growth of E. coli WT MG1655 and the respiratory variants was performed in their respective evolution media, i.e. minimal media supplemented with succinate (2g/L sodium succinate dibasic) and glycerol (0.2%) as the carbon source. The strains were grown at 37C in two biological replicates for each strain. Total RNA was extracted from the mid-log phase culture for RNA-seq library preparation.