Expression data from KKAy mice ileum tissue treated with voglibose

Voglibose is an α-glycosidase inhibitor with anti-inflammatory effects on the intestine, but the underlying mechanism is not clear. Our study defined the dynamic changes of the differentially expressed genes after treatment of voglibose in diabetic KKAy mice, and provide basis for comprehensive understanding of the disease at molecular level. We investigated gene expression alteration after a long-term treatment of voglibose in diabetic KKAy mice. After feeding for 4 weeks of high-fat diets, KKAy mice were treated intragastrically with Voglibose (1mg/kg) or an equivalent volume of water (0.05 ml/10 g body weight) once daily for 8 weeks. Total RNA was extracted from ileum tissues using Trizol reagent (Life Technologies, Carlsbad, CA, USA) (n=4 per group) according to manufacturer’s instructions. All arrays were scanned by using Affymetrix® GeneChip Command Console (AGCC) which was installed in GeneChip® Scanner 3000 7G. A total of 2,2207 detectable effective genes were obtained and 1528 genes were significantly differentially expressed genes (fold change > 1.2, p < 0.05) between the voglibose-treated group and the DM group, of which 603 were upregu-lated and 925 were downregulated.