Gene expression data of 2D duodenum organoids with or without continuous flow. Gene expression data of 2D duodenum organoids with or without continuous flow

We developed a monolayer culture system for small intestinal organoids, and then placed the culture plate on a rotator to generate a constant flow in the overlay medium. This mechanical stimulation facilitated the formation of villus-like structures from the monolayer of small intestinal organoids. Overall design: We analyzed gene expression of 2D normal duodenum organoids with or without medium rotation. Total RNA was extracted from organoids with or without a four-day medium rotation, and after 0, 1, 2 or 4 days of medium rotation, and sequenced with HiSeq X Ten (Illumina). *** RNA sequencing controlled-access data have been deposited to the Japanese Genotype-phenotype Archive under the accession number JGAS000256. ***