Transposase-mediated integration events of ‘solo’ substrates.

HeLa cells were co-transfected with the ‘solo’ transposon constructs in the present of either mPB or SB100X transposases, while a catalytically inactive SB transposase, D3 was used as a control. Frequency of substrate integration was calculated as a ratio of colony numbers in the presence vs absence of transposases. Colonies were picked and analysed for transposase-mediated integration events. Transposase-mediated integration is defined when the IR of the transposon is integrated into a respective target site in the genome (see also Supporting Text S1). ND: not detected.