Clara Franzini-Armstrong (2011) CIL:18450, damselfly, flight muscle cell. CIL. Dataset

Most images show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images. This image is part of the group image CFA00512. TEM magnifications is not available for this image. The negative film was scanned at LBL then reduced in size for upload. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron. Technical details: Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer. pH 7.2. stored at 4C for several days. Muscle dissected out. Infiltrated in 30% glycerol in water. Frozen in liquid nitrogen cooled Freon, fractured in Balzers 400 Freeze-fracture, shadowed with platinum at 45o angle. Replicas were cleaned with Clorox (to remove tissue and with either sulfuric acid or NaOH to try removing the tracheolae. Replicas examined in a Philips 410 Microscope. Reference for these images:
Takekura, H. and Franzini-Armstrong C. The structure of Ca2+ release units in arthropod body muscle indicates an indirect mechanism for excitation-contraction coupling. Biophys. J. 83, 83, 2742-2753, 2002.

多数图像展现了线粒体外膜及其嵌于线粒体表面长沟中的二联管SR/T的断裂情况。膜的所有瓣叶均在图像中呈现。该图像属于CFA00512图像组的一部分。本图像的透射电子显微镜放大倍数不可用。负片在LBL进行扫描后，为便于上传而进行了尺寸缩减。扫描步长为6.3微米，上传时缩小4倍，有效像素尺寸为25微米。技术细节：去除头部和尾部，将胸部分为两半并浸泡于0.1 M草酸盐缓冲液中的3%戊二醛缓冲液中，pH值为7.2，于4°C保存数日。肌肉组织被分离出来。在水中浸泡于30%甘油中。在液氮冷却的氟利昂中冷冻，并在Balzers 400冷冻断裂机上断裂，以45度角进行铂阴影处理。复制品用漂白剂（以去除组织）以及硫酸或NaOH（以尝试去除气管）进行清洗。复制品在Philips 410显微镜下进行检查。图像参考：Takekura, H. 和 Franzini-Armstrong C. 《节肢动物身体肌肉中Ca2+释放单位的结构表明了兴奋-收缩偶联的间接机制》。生物物理学杂志，第83卷，第2742-2753页，2002年。