Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of Interphase COS-7 cell (jrc_cos7-1a)

<b>Sample</b>: Wild-type, interphase COS-7 cell (ATCC CRL-1651)<b>Sample Description</b>: Near isotropic FIB-SEM imaging of cultured, wild-type, interphase COS-7 cell (ATCC CRL-1651). Imaging acquisition reconstructed using Hess Lab software.<b>Protocol</b>: High pressure freezing, freeze-substitution resin embedding with 2% OsO₄ 0.1% UA 3% H₂O in acetone; resin embedding in Eponate 12.<b>Contributions</b>: Sample provided by Melanie Freeman (Infinimmune) and David Hoffman (10x Genomics), prepared for imaging by H. Amalia Pasolli (Rockefeller), imaging by C. Shan Xu (Yale), with post-processing by Gleb Shtengel and Harald Hess(HHMI/Janelia).<b>Acquisition ID</b>: jrc_cos7-1a<b>Voxel size (nm)</b>: 4 x 4 x 4 (x, y, z)<b>Data dimensions (µm)</b>:<b> </b>25.4 x 6.0 x 4.1 (x, y, z)<b>Imaging start date</b>: 2016-11-05<b>Imaging duration (days)</b>: 7<b>Landing energy (eV)</b>: 400<b>Imaging current (nA)</b>: 0.25<b>Scanning speed (MHz)</b>: 0.400<b>Dataset URL</b>: s3://janelia-cosem-datasets/jrc_cos7-1a/jrc_cos7-1a.zarr/recon-2/em/<b>Visualization Website</b>: https://openorganelle.janelia.org/datasets/jrc_cos7-1a<b>Publication</b>: Xu et al., 2017; Xu et al., 2021