Evaluation of the TRF immunoassays using Syphilis Mixed Titer Performance Panel (PSS202).

a Assays performed using commercial kits as indicated. Results were provided by the panel supplier. RPR, rapid plasma reagin; ATA, anti-Treponema antibody; S/Co, signal to cutoff ratio; EIA, enzyme immunoassay; TPPA, Treponema pallidum particle agglutination assay; TPHA, Treponema pallidum haemagglutination assay; Neg, negative; R, reactive; NR, non-reactive. RPR results are endpoint dilutions. S/Co ratios ≥1.0 are considered reactive.
b Values indicate S/Co ratios obtained in this study, using the in-house TRF immunoassays with 10 min and 1 h incubation times. Samples with S/Co values <1.0 are designated as negative (−) and those with values ≥1.0 are designated as positive (+).