Single cell transcriptomic profiling of trunk tissue isolated from spinal cord-lesioned control zebrafish larvae or following depletion of pdgfrb+ cells

The goal of this study was to map the transcriptional changes with or without depletion of pdgfrb+ cells after spinal cord lesion in zebrafish larvae at single cell level. Overall design: RNA profiles of single cells isolated from trunk tissue of spinal cord-lesioned Tg(BAC(pdgfrb:Gal4ff))ncv24;Tg(UAS-E1b:Eco.NfsB-mCherry)c264 (referred to as pdgfrb:NTR-mCherry) transgenic zebrafish larvae, treated with Metronidazole or DMSO control, were generated by scRNA-seq, in duplicates, using Illumina NovaSeq 6000