Raw spectral data - SWATH-MS of Cannabis sativa glandular trichomes in response to abscisic acid and drought stress

Cannabis (Cannabis sativa L.) is a medicinally important drought resistant plant. The glandular trichomes (GT) on female flowers of cannabis are the main site of production and storage of cannabinoids and terpenoids. While water deficit stress (WD) modulates cannabinoid content in cannabis, it is unknown how GT regulation is affected to achieve this. We quantified the GT proteome and floral cannabinoid and terpenoid content in late flowering ‘Hindu Kush’ cannabis treated with either short-term WD or with an abscisic acid (ABA) foliar spray. We found that WD and ABA changed the abundance of many of the same proteins in the GT, with changes reflecting a decrease in primary metabolic processes and an increase in stress response mechanisms. Results suggested ABA likely mediates changes in the GT indirectly by affecting salicylic acid and ethylene signalling. Interestingly, WD caused cannabinoid content to increase, while ABA caused monoterpene content to increase, and proteins involved in cannabinoid or terpenoid biosynthesis were either downregulated or did not change in either treatment. Since photosynthesis and the total photoassimilate pool typically decline in response to WD in plants, we postulate that the remaining photoassimilate pool is reallocated to support secondary metabolism in the Cannabis GT when responding to WD.