Proteoglycan 4 (lubricin) is a highly sialylated glycoprotein associated with cardiac valve damage in animal models of infective endocarditis

Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of tooth surfaces and are generally associated with oral health, but can also cause infective endocarditis (IE). These species express “Siglec-like” adhesins that bind sialylated glycans on host glycoproteins, which can aid the formation of infected platelet–fibrin thrombi (vegetations) on cardiac valve surfaces. We previously determined that the ability of S. gordonii to bind sialyl T-antigen (sTa) increased pathogenicity, relative to recognition of sialylated core 2 O-glycan structures, in an animal model of IE. However, it is unclear when and where the sTa structure is displayed, and which sTa-modified host factors promote valve colonization. In this study, we identified sialylated glycoproteins in the aortic valve vegetations and plasma of rat and rabbit models of this disease. Glycoproteins that display sTa vs. core 2 O-glycan structures were identified by using recombinant forms of the streptococcal Siglec-l..., , # Proteoglycan 4 (lubricin) is a highly sialylated glycoprotein associated with cardiac valve damage in animal models of infective endocarditis

Dataset DOI: [10.5061/dryad.73n5tb384](10.5061/dryad.73n5tb384)

This data set was used to compare the levels of PRG4/lubricin in the plasma of 8 rats in an animal model of infective endocarditis. Plasma samples were collected from each animal at 3 time points: a) pre-surgery; b) 3-days after placement of a trans-aortic valve catheter; c) 3 days after infection with Streptococcus gordonii.

DATA & FILE OVERVIEW

1. File list:

A) rats171009_2abc3abc4abc_PRG4western.tif
B) 1710093a_rats180115_1abc2abc3abc_PRG4western.tif
C) 1710093a_rats180226_1abc2abc_PRG4western.tif
D) Fig4A_ImageJ_MeasuredValues.csv
E) Fig4A_NormalizedValues.csv

1. Relationship between files: The .csv files contain data gathered from the three .tif files.

## DATA-SPECIFIC INFORMATION FOR: rats171009\_2abc3abc4abc\_PRG4western.tif

Lane 1: rat1710092 condition a
Lane 2: rat1...,