Mutations that suppress the temperature sensitive phenotype caused by dnaBK85E (dnaB134ts).

1All strains are derived from KPL69 and contain the indicated suppressor, dnaB134ts (dnaBK85E) and the linked zhb83::Tn917 (mls).2One representative strain is indicated, along with the total number of independent isolates that were sequenced. In addition, the second number in parentheses indicates the total number of mutants with a phenotype indistinguishable from that of the sequenced representatives.3The suppressor mutation in this strain appears to be in the rrnO operon (which includes trnO-ala and trnO-ile). There were four suppressors in this class. The suppressor mutation in the strain indicated (MER498) was linked to the rrnO operon and a clone of the operon suppressed dnaB134ts when integrated into the genome. The suppressors in the other three strains were neither cloned nor tested for linkage. The sequence of each of the two tRNA genes in all four suppressors was wild type, indicating that the mutation, at least the one cloned, is most likely in an rRNA gene in the rrnO operon.