RNA-Seq from human innate lymphoid cells (ILCs) transitional populations.

Innate lymphoid cells (ILCs) are tissue-resident lymphocytes subdivided into ILC1s, ILC2s and ILC3s based on core regulatory programs and signature cytokines secreted. ILCs exhibit functional plasticity. Using flow cytometry, mass cytometry and scRNAseq, here we found that ILC3s and ILC1s occupy opposite ends of a spectrum including discrete subsets in human tonsils. The human intestine also exhibited an ILC3–ILC1 transitional population. Overall design: ILCs were sorted from human tonsils and human intestine and analysed by scRNA-seq and bulk RNA-seq. The sc-RNAseq sequencing data is available upon reasonable request to the authors.