The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8 T cell exhaustion

Table S1: Gene Set Enrichment Analysis (GSEA) of the oncogenic signature gene sets (MSigDB C6 gene set) enriched in Tex versus Teff cell clusters. RNA-seq data are mined from GSE89307, GSE84820, and GSE86881. Refer to first tab of excel sheet for detailed description of column headings in subsequent data tab.

Table S2: qPCR, shRNA and sgRNA oligonucleotide sequences. 5’ to 3’ oligonucleotide sequences used for qPCR, CRISPR-Cas9 mediated knockout, and shRNA-mediated knockdown. Q denotes primers used for qPCR, and F and R denote forward and reverse primers, respectively.

Table S3: Differential gene expression analysis from RNA-seq of adoptively transferred Keap1-/- versus WT P14 cells, isolated 7 days post LCMV Armstrong infection. Refer to first tab of excel sheet for detailed description of column headings in subsequent data tab.

Table S4: C2, C5, C6, C7, NFE2L2.v2 and H Gene set enrichment analysis (GSEA) from RNA-seq of Keap1-/- versus WT P14+ CD8+ T cells upon LCMV Armstrong infection. A Positive or negative normalized enrichment score (NES) indicates a gene set enrichment in Keap1-/- cells or WT cells, respectively. Refer to first tab of excel sheet for detailed description of column headings in subsequent data tabs.

Table S5: Differential gene expression analysis from RNA-seq of in vitro cultured CD8+ T cells with variable expression of PTGIR, treated with vehicle or iloprost. Refer to first tab of excel sheet for detailed description of column headings in subsequent data tabs.

Table S6: Pathway analysis from RNA-seq of in vitro cultured CD8+ T cells with variable expression of PTGIR, treated with vehicle or iloprost. Refer to first tab of excel sheet for detailed description of column headings in subsequent data tabs.

Liver annotated blots: Original western blot (labelled) for liver cell western blots show in Figure 1D

HO1_LAMINB1_Tcells- Original western blot (labelled) for HO-1 and LAMIN B1 T cell western blots show in Figure 1D.

KEAP1_Tcells- Original western blot (labelled) for KEAP1 T cell western blots show in Figure 1D.

NQO1_Tcells- Original western blot (labelled) for KEAP1 T cell western blots show in Figure 1D.