RUNX1 overexpression in precursor B acute leukemia cells

Overexpression of the transcription factor RUNX1 was studied in Nalm-6 precursor B acute lymphoblastic leukemia cells. The active enhancer histone marker H3K27ac and RUNX1 were measured using ChIP-seq. The effect on gene expression was analyzed using single cell RNA-sequencing. Overall design: ChIP-seq profiles were generated from Nalm-6 pre-B-ALL cell line after induced wt RUNX1 or empty vector expression for 48 h. Empty vector induction cell line was used as controls. Mnase digestion was used for DNA fragmentation. Antibodies against H3K27ac and RUNX1 compared to input. scRNA-seq samples were generated from 72 h after induction using the 10X Genomics Chromium platform.