Effect of Ifnar1 Knockdown in a mouse model of Cerebral Interferonopathy

To investigate effect of antisense oligonucleotides targeting the murine IFNa receptor (Ifnar1 ASOs) in a transgenic mouse model of cerebral interferonopathy (GIFN), mice were injected intracerebroventricularly with Ifnar1 ASOs. We performed DGE-Seq on brain cortex samples to determine the impact of ASOs on the transcriptome. Treatment of GIFN mice with Ifnar1 ASOs resulted in a significant decrease in Interferon gene expression and dampened the hyperinflammatory transcriptomic landscape in the brains of GIFN mice. Overall design: Comparative gene expression profiling analysis of transcriptome sequencing data for cortex samples from wildtype and transgenic mice treated with Ifnar1 ASOs or Vehicle.