Next Generation Sequencing for Quantitative Transcriptomic Profiling of TLR3, TLR7 and TLR8 Activation in Neurons

The goal of this study is to compare gene expression of mouse cultured cortical and hippocampal neurons in response to TLR3, TLR7 and TLR8 activation Overall design: At 4 day in vitro, wild-type mouse cortical and hippocampal neurons were treated with vehicle control, poly IC (for TLR3 activation) and CL075 (for TLR7 activation). Tlr7-/- neurons were treated with vehicle control and CL075/poly dT (for TLR8 activation). Six hr after treatment, total RNAs were extracted for deep sequencing, in triplicate, using the NextSeq500 High-output v2 (75 cycle) sequencing kit on the Illumina NextSeq500 instrument.