Analysis of small extracellular vesicle derived miRNAs using high through-put miRNA sequencing

To gain insight into the microRNA expression profile of small extracellular vesicle derived from different cell types and to verify their mechanism, we utilized the miRNA sequencing technology to analyze the miRNA profiles of different human cell derived small extracellular vesicle We collect the small extracellular vesicles from the supernatant of several widely used stem cell types and osteoblst using ultracentrifugation method. The small extracellular vesicles were then extracted for small RNAs to perform high through-put sequencing