Integrin αDβ2 is Dynamically Expressed by Human Monocyte-derived Macrophages (MDM) and Dendritic Cells (MDDC).
收藏Figshare2015-12-02 更新2026-04-29 收录
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Isolated human monocytes were cultured in human serum (Experiment 1) or M-CSF (Experiment 2) for 3 days to induce differentiation into monocyte-derived macrophages [28],[29]. After 6 days in culture they were treated with LPS, with IFNg as a stimulus for “classic activation”, or with IL-4 as a stimulus for “alternative activation” [46],[47]. In a third experiment monocytes were cultured in GM-CSF and IL-4 to induce differentiation to immature MDDC (“control”); they were then treated with LPS to trigger transition to mature MDDC (“LPS”) [29]. Results are expressed as mean fluorescence intensity (MFI), with the percentage of positive cells in parentheses.Integrin αDβ2 is Dynamically Expressed by Human Monocyte-derived Macrophages (MDM) and Dendritic Cells (MDDC).
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2015-12-02
