Sperm transcriptome of Crassostrea gigas

The risk of norgestrel (NGT) exposure in the aquatic environment has been identified, but the toxic effects on invertebrate germ cells are unknown. Therefore, the toxic effects of NGT on oyster sperm were investigated by combining transcriptome.Transcriptome sequencing showed that differential genes were significantly enriched in regulation of cellular processes, protein digestion and absorption signaling pathways, and focal adhesion. We found that NGT exposure caused significant decreasing of mitochondrial ATP binding cassette and calcium uptake protein 1, and triggered apoptosis in sperm. This study is helpful to reveal the toxic effect of water environment NGT on in vitro fertilization invertebrate germ cells, and provide theoretical basis for formulating relevant environmental protection measures.