Additional file 1 of Rivers as a potential dispersing agent of the invasive tree Acacia dealbata

Table S1. Geographic location of each sampled point (WGS 84 UTM zone 19S), with its hydraulic characterization, captured pods and samples, and other estimated variables. Figure S1. Summary of germination experiment. A, Firstly, to evaluate the probability of germination while drifting in the river's flow, we conduct ‘germination in water’ experiments. Using the immersion device shown in Fig. 2, we simulate transport by the river of both scarified and unscarified seeds. We assess the effect of immersion duration (15, 35, and 55 days). As a control treatment, we germinate seeds in water without any movement. B, Secondly, to assess germination post river transport (post deposition), we perform a ‘germination over substrate’ experiment with those unscarified seeds previously used in the ‘germination in water’ experiments with motion (Fig. 2), scarifying half of them. Again, we evaluate the effect of immersion duration in water in movement (15 days, 35 days, and 55 days). Some seeds rotted during the ‘germination in water,’ which was quantified; this decreased the number of samples for the ‘germination over a substrate.’ As control treatment, seeds that were previously not immersed in water were also tested. Figure S2. Schematic drawing of the device used for continuous immersion and motion of the seeds, in the ‘germination in water’ experiments. A) A small electrical motor with a gearbox and a propeller is attached to a wooden support. B) Each seed is placed in a pocket, which is suspended from a wooden frame that is attached to the propeller. C) The frame is placed atop a plastic container filled with water. The circular motion of the electrical motor generates a vertical up-and-down movement, maintaining the seeds in continuous motion. Figure S3. Relationship between the Reynolds number and A. dealbata’s seeds caught in the river flow (seed/min), at each sampling site. Linear regression is significant (R2 =0.363, p =0.008).