Fluorescence emission spectra of Bovine serum albumin (BSA), Thermomyces lanuginosus lipase (TLL), Candida rugosa lipase (CRL).

Figure 1. Fluorescence emission spectra (AU, arbitrary units) of four different proteins [Bovine serum albumin (BSA), Thermomyces lanuginosus lipase (TLL), Candida rugosa lipase (CRL)]. Data for proteins in aqueous buffer (10 mM Tris-HCl, pH 7.0) and lyophilized protein powders are given. All these fluorescence emission spectra were recorded with excitation at 280 nm using excitation and emission slit widths of 2 nm and 5 nm, respectively.