Sediments of Ologe lagoon were collected from three locations based on the nature of anthropogenic pollution it receives. The sites include Ologe human activity site (OLHAS), Ologe industrial contaminated site (OLICS) and Ologe presumed relatively undisturbed site (OLPS). Sediments were collected and pooled within an area of 10 m sq and physicochemistry of the samples were determined following standard analytical procedure. Total DNA were extracted using ZymoBIOMICS DNA extraction kit for sediment (Catalog No. D4300T, ZYMO RESEARCH) and sequenced usingIllumina Next Generation Sequencer (NGS). Illumina raw sequence were trimmmed using Trimmomatic while bacteria community strucure of the sediments were analyzed via EDGE Bioinformatic.Statistical analysis of the metagenomic read was done using statistical analysis for metagenomic profile (STAMP), Graphpad. Heatmap was done using XLSTAT, principal component analysis was done using PRIMER (version 7).. Metagenomic insight into microbial com

Ologe lagoon an inland waterbody in Lagos state Nigeria, is one of the lagoons witnessing high level of anthropogenic activities from diverse source. These has made the sediments the ultimate sink for various pollutants. The lagoon sediments were divided into three sections based on the nature of imputes received, which include Ologe industrial contaminated sites (OLICS), Ologe human activity sites (OLHAS) and the presumed relatively undisturbed sites (OLPS). Sediments from each site were collected and pulled to form a composite. The physicochemistry of the sediments were carried out via standard analytical procedure. The pH of the sediment was 4.190 ± 0127, 5.010 ± 0.014 and 4.715 ± 0.027 for OLHAS, OLICS and OLPS respectively, the total dissolved solids (mg/L) were 8785.141 ± 22.486, 8288.891 ± 123, 8611.750 ± 25.456 respectively. The nitrogen contents were 44.380 ± 0.962, 59.485 ± 0.827 and 7.440 ± 0.085 for OLHAS, OLICS and OLPS. Heavy metal contents of the sediments were determined. Total DNA from the sediment were extracted using DNA extraction kit and sequenced using hiseq Illumina Next Generation Sequencer. The alpha diversity ACE, Chao1, Jacknife, Shannon, Simpson, NPShannon and phylogenetic diversity of the microbial community structure of each site were determined. The structural analysis of the metagenomes was done via EDGE Bioinformatics. At phylum level, 43 phyla each were retrieved from each metagenome from each site and 75 classes each respectively. Also 165, 161 and 166 bacteria Orders were retrieved from OLHAS, OLIC and OLPS. Using read base analysis 340, 334 and 334 families were delineated across the three sites. At Genus level 986, 927 and 866 bacteria genera were retrieved. At species level 1476, 1129 and 1327 members were obtained, while 1059, 810 and 913 bacteria strains were retrieved from OLHAS, OLICS and OLPS metagenomes. Unclassified Unranked groups were also retrieved. From OLHAS metagenome, the abundance of each phylum was Proteobacteria (43.5 %) Firmicutes (16.12 %), Actinobacteria (7.90 %), Chloroflexi (5.38 %), Bacteroidetes (4.65 %), Planctomycetes (3.29 %) Nitrospirae (2.8 %), Acidobacteria (2.70 %), Euryarchaeota (2.14 %). OLICS metagenome the phyla abundance was Proteobacteria (59.85 %), Actinobacteria (9.96 %), Firmicutes (8.94 %), Chloroflexi (5.32 %), Acidobacteria (1.87 %), Planctomycetes (1.83 %) Spirochaetes (1.73 %), Bacteroidetes (1.58 %), Nitrospirae (1.19 %), Verrucomicrobia (1.17 %) and Euryarchaeota (0.80 %) while phyla abundance in OLPS metagenome was Proteobacteria (41.36 %), Firmicutes (14.65 %), Actinobacteria (9.61 %), Chloroflexi (7.62 %), Nitrospirae (4.14 %), Planctomycetes (3.41 %), Acidobacteria (3.35 %), Spirochaetes (1.92 %), Bacteroidetes (1.61 %), Euryarchaeota (1.58 %), Verrucomicrobia (1.43 %), Gemmatimonadetes (1.24 %), Cyanobacteria (0.80 %). The statistical analysis of the metagenomes was analyzed using STAMP, PRIMER , XLSTAT, Graphpad,Tand Microsoft excel software. The metagenomic insight into this inland lagoon had revealed a wide range of organism with diverse biotechnological application. Keywords: Next Generation Sequencing, Metagenome, Microbial community structure, Alpha diversity, Proteobacteria, Firmicutes, Actinobacteria.