Effect of inhibition of endocytosis on transcytosis of DNA-fragment (5 nM).

Transcytosis of DNA fragments and Lucifer yellow (LY) in the apical to basolateral direction were quantified as described in Materials and Methods and tested statistically with a linear mixed-effects model. Inhibition factor is calculated as control divided by treated. The number n is the number of independent experiments and the number m is the total number of wells analysed (observations). Control for ice-treatment is 37 °C and control for bafilomycin A1 (BafA1)-treatment is with vehicle (DMSO). TEER = trans-epithelial electric resistance.