Plant growth promoting bacteria. Plant growth promoting and anti-plant pathogenic effects of bioformulation

Excessive use of chemical fertilizers, insecticides and pesticides in agriculture over the years resulted into poor soil quality and fertility. Chemical insecticides and anti-plant pathogenic compounds are xenobiotic. These have bioaccumulation effect and thus have adverse impact on the food chain. due to which Ssustainable approaches for making fertilizers are increasingly becoming popular. In this study we made an attempt to make a bioformulation having plant growth promoting and anti-plant pathogenic properties. Bacteria such as Azotobacter, Rhizobium and Pseudomonas aeruginosa having plant growth promoting (PGP) properties were isolated from soil sample using congo red yeast extract mannitol agar and Ashby's agar. Pseudomonas aeruginosa showed production of maximum plant growth promoting substances such as Indole acetic acid, HCN (hydrogen cyanide), siderophores, ammonia, nitrate reduction, phosphate solubilisation property and exoplysaccharide (EPS) production. Different Various biofertilizer formulations (BIOFERT-Azo, BIOFERT-Rhizo, BIOFERT-Pseudo and BIOFERT-RAP) were prepared as mentioned in the literature using above PGP bacteria along withand different plant leaves known to have anti-insecticidal and anti-plant pathogenic properties. Total nitrogen, phosphorus and ammonia content in the bioformulation were determined. BIOFERT RAP was having theshowed highest content for of N, P and NH3 which was 0.337 %, 442.58 mg/l and 99.4mg/l respectively. Pot experiment was carried out to study the Eeffect of various BIOFERT on the growth of the Vigna radiata (mung bean) in comparison with negative control and positive controls having (commercial PGP substances- Agrilabh-RIZ and Agrilabh-AZO, vermicompost and diammonium phosphate (DAP).