Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Mock Pilot

The HMP Sanger mock pilot represents the dideoxy sequencing of 16S rRNA genes amplified from HMP even and staggered Mock communities, distributed to each of the four HMP sequencing centers. The goal of the pilot was to test the provisional Sanger sequencing protocol and to evaluate accuracy and consistency between centers.