* Note: this is a serendipitous mutation introduced by PCR during the generation of the M121C mutant. Both the M121C/T169M double mutant and the single mutants were characterized.Site directed mutants
ACP mutants number 3 to 149 were obtained by random mutagenesis. Mutants ACP745 and ACP749 were obtained by site-directed mutagenesis. Mutant ACP(S36T) was described previously [15]. Functionality cor
aOrder of mutants listed matches the ordering in Figures 1 and 2.bORF numbers, gene names, and descriptions are from the National Microbial Pathogen Data Resource web site (www.nmpdr.org).cPercentages
