Altered cartilage gene expression in Aga2 OI mouse negatively impacts linear growth through Sox9 and FGF signaling

Osteogenesis Imperfecta is characterized by short stature however the cellular mechanisms behind this phenotype are unclear. We isolated tibial and femoral cartilage growth plate chondrocytes from postnatal day 5 wild type and Aga2 (a model of OI) mice and analyzed differential expression patterns using single cell RNA-seq Growth plate chondrocytes from Wild-type and Aga2 mice were isolated from femoral and tibial cartilage tissue at postnatal day 5 and analyzed using scRNAseq.