Table 2_Bulk RNA-seq datasets analysis integration identifies robust drought-responsive genes and functional networks in Eucalyptus grandis.xlsx

IntroductionEucalyptus grandis is a cornerstone of global forestry, yet its productivity is increasingly threatened by drought. Understanding the molecular mechanisms underlying drought response is essential for improving resilience.
MethodsWe conducted a meta-analysis of three independent RNA-Seq drought studies in E. grandis, applying a rigorous bioinformatic pipeline with leave-one-out Jackknife validation to ensure robustness and reduce single-study bias.
ResultsWe identified a high-confidence set of 472 differentially expressed genes (DEGs), including 274 upregulated and 198 downregulated genes. Functional analysis revealed a growth-defense tradeoff, with upregulated genes associated with stress response pathways such as protein folding, osmolyte biosynthesis, and ABA signaling, while genes involved in cell division, DNA replication, and cell wall biosynthesis were repressed. Protein–protein interaction analysis showed a coordinated network linking stress response activation to suppression of growth-related processes.
DiscussionThese findings provide a robust catalog of candidate genes, including previously uncharacterized proteins, supporting future functional studies and molecular breeding strategies aimed at enhancing drought tolerance in eucalyptus under climate change.