Upstream regulator of genomic imprinting in rice is a small RNA-associated chromatin remodeler [RNA-seq]

Knock down of CLSY3 in rice caused endosperm development defects. We investigated the molecular effects of loss of CLSY3 in rice endosperm by profiling sRNA and mRNA transcriptomes .These analyses revealed the roles of CLSY3 in Endosperm development. Overall design: We performed RNA seq analyses to look at the expression of the genes in WT and clsy3 kd at 20 days after anthesis. We performed RNAseq analyses to look at the expression of the genes in embryo and endosperm 25days days after anthesis.