Bovine monocyte derived macrophages from peripheral blood

Resistance traits of parasitic infestation and diseases in bovine breeds display various unknown differences in the immune response indicating to be related to the host`s genomic background. We studied the differential gene expression of monocyte-derived macrophages (MDMs) stimulated or unstimulated with LPS in vitro, from two bovine breeds by RNA sequence methodology. MDMs were grown in supplemented RPMI medium for 11 days. Then, 100 ng/ml LPS was added to medium for 48h in treated samples. Total RNA was extracted from macrophages and sequenced by RNA-Seq technology. Resulting sequences were mapped to the bovine reference genome and gene expression was compared between breeds. We found several differences in immune response activation, as leucocyte recruitment, immune response, antigen processing and presentation, positive regulation of NF?B and cell cycle biological processes in unstimulated cells and lipid metabolism, inflammatory response, regulation of cell proliferation and cycle and arginine catabolism in LPS treated cells. Finally, LPS seems to trigger inflammation by distinct pathways in these two bovine breeds. Overall design: Comparison between bovine primary monocyte derived macrophage cultured cells from two differrent breeds treated and not-treated with LPS for 48h