Retrospective analysis of mesendoderm progenitors that give rise to cardiac microtissues, multilineage and gut organoids. Retrospective analysis of mesendoderm progenitors that give rise to cardiac microtissues, multilineage and gut organoids

Bulk RNA-sequencing of the mesendoderm progenitors before aggregation. Mesendoderm progenitors aggregates can lead to the formation of cardiac microtissues, multilineage and gut organoids in different batches. Despite batch-to-batch differences in spheroid phenotype, all spheroids (n>200) within a single batch derived from the same initial differentiation of progenitor cells manifested identical morphologies and cellularity. Overall design: Mesendoderm progenitors at differentiation day 5.