Hepatocytes :MH vs. ProliHH vs. PHH vs. HLC

Hepatocytes isolated from DILI patient's liver (#2064) were cultured for a long term using irrMEF and EMUKK-05, and comprehensive gene expression was compared between Puromycin-treated and non-treated groups. In addition, comprehensive gene expression analysis of human mature hepatocytes, primary cultured cells, and ips cell-derived hepatocyte-like cells were performed as controls. Two-condition experiment, Proliferating hepatocytes (ProilHH) vs. puromycin-treated ProliHH. Primary human hepatocytes (PHH) and isolated humen mature hepatocytes (MH) and human iPSC-derived hepatocyte-like cells (HLC) as controls. Hepatocytes were isolated from the livers of human DILI patients and frozen after primary culture. The cells were cultured for a long turm of time in MEF feeder and EMUKK-05 medium (ProliHH), and the gene expression changes between the puromycin- and non-puromoted groups were analyzed. Puromycin was used as a selective agent for hepatocytes.