Effect of 4-octyl itaconate on gene expression of dendritic cells

Here, we report that macrophage-derived itaconate exerts a significant suppressive effect on dendritic cell (DC) function. To delve deeper into the impact of itaconate on DCs, we treated bone marrow-derived dendritic cells with 4-OI, a derivative of itaconate, and conducted RNA-seq analysis. Gene-set enrichment analysis (GSEA) of 4-OI-treated DCs showed a general downregulation of innate immunity and immune-response pathways. To further investigate the effect of itaconate on DCs, we performed RNA-seq analysis on bone marrow-derived dendritic cells (BMDCs) that were either incubated with IFN-γ alone or co-treated with IFN-γ and 4-OI. We then performed gene expression profiling analysis using the RNA-seq data obtained from BMDCs of IFN-γ group and IFN-γ+4-OI group. Comparative gene expression profiling analysis of RNA-seq data for BMDCs of IFN-γ group and IFN-γ+4-OI group.