Predicted distances (Å) between Cα atoms of the same or different residue pairs in NBD dimers of the half transporters LmrA, Sav1866, and MsbA.

The LmrA residue pairs used in this cross-linking study are identified in the first column; pairs for which clear cross-linked products were observed are shown in bold font. The equivalent pairings for Sav1866 and MsbA and the predicted distances between the residue pairings are given in columns 2–5. The indicated residue numbers correspond to the primary sequences identified in the alignment (Fig 1).
a Distances for LmrA S361 in the sandwich dimer were estimated by structural alignment of the LmrA NBD structure (PDB: 1MV5) and the Sav1866 structure (2HYD), using Cα atoms of the NBD core subdomains (LmrA residues 342–352, 358–406, 500–505, 530–536, and 545–561) and measuring from LmrA S361.
b Distances for LmrA S361 in the inward-facing closed conformation were estimated by measuring distances from K354 in V. cholerae MsbA (3B5X).
Predicted distances (Å) between Cα atoms of the same or different residue pairs in NBD dimers of the half transporters LmrA, Sav1866, and MsbA.