Cdk5i peptide treatment in aged Tau P301S mice

To investigate the effects of Cdk5i peptide treatment on gene expression in cortical neurons that isolated from aged Tau P301S mice We began delivery of either Cdk5i or scrambled peptides every other day by IP injection (20 mg/kg body weight) to a group of Tau P301S mice aged 7 to 9 months old. We isolated NeuN+ nuclei from cortical tissue of 7- to 9-month-old animals for downstream RNA-seq analysis.