HP1γ is required for G9a/GLP coactivator function with the glucocorticoid receptor in Nalm6 cells. HP1γ is required for G9a/GLP coactivator function with the glucocorticoid receptor in Nalm6 cells

Using a genome-wide analysis, we demonstrated that HP1γ is selectively required for GC-regulated expression of GR target genes that require G9a and GLP, consistent with our previous finding that automethylated G9a and GLP recruit HP1g to specific genomic GR binding sites. Overall design: We examined whether HP1g is required for GC-induced expression of the genes that require coactivators, G9a and GLP, by depleting HP1g, G9a and GLP in Nalm6 cells using shRNA and examined gene expression changes at 8 hour dexamethasone (dex) treatment compared with ethanol treatment as a control. shRNA for a nonspecific sequence (shNS) followed by 8h of dex treatment was performed as control.