Morphological and specific marker characterization of fibroblast-derived IPSC.
收藏Figshare2016-02-23 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Morphological_and_specific_marker_characterization_of_fibroblast_derived_IPSC_/1607765
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(A) Fibroblasts from a male healthy or patient with AMN or cALD disease were transduced with retroviral vectors expressing reprogramming factors OCT4, SOX2, NANOG, LIN28, KLF4, and c-MYC as described under methods. IPSC colony before isolation. (B) A putative control IPSC line was isolated and expanded under feeder-free maintenance medium for human IPSC, colonies growth was observed for 5 days by phase contrast image. (C-D) Control, AMN or cALD IPSC expressed the SOX2 and SSEA4 markers of pluripotency. (E) Summary chart depicts the markers for IPSC lines that were characterized. Scale bars represent 200 μm.
创建时间：
2016-02-23
