Supplemental Material for Boone et al., 2020
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Table S1 Primers
for strain construction and confirmation. Primers
for DJ-PCR-based fluorescent tagging were designed as previously described
(Hammond et al. 2011b). The center DJ-PCR
fragment for nup120-mCherry tagging
was obtained from plasmid pEB1.1, which is similar to pTH1112.8 except that mCherry (Castro-Longoria et al. 2010) is present instead of yfpn. The center DJ-PCR fragment for yfpn-cbp20 and yfpn-sad-8 tagging was obtained from plasmid pNTYN-2, which is
similar to pTH1123.1 except that nat1
(Lichius and Read 2010) is present instead of hph.
创建时间：
2020-04-14
