Childia transcriptome

Childia a flatworm is a primitive organism of the phylum Platyhelminthes in the class Acoela. Here, using pyrosequencing approach we initiated transcriptome analysis of this organism. Specifically, we developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis. Combined 5’ with 3’ targeted libraries produces greater coverage of individual transcripts and high quality assembly.