dmLT_Fig. 2. Recovery of intact dmLT after incubation in saliva

Double mutant heat labile (dmLT) adjuvant is being proposed for oral vaccine formulation. dmLT stability in saliva was evaluated by dmLT ELISA. dmLT stability was tested using two separate preparation methods to prevent any interference with saliva and the ELISA method. In the first method dmLT was mixed with saliva in tubes, incubated at 37˚C, and tested at 5, 10, 30 minutes by ELISA. In the second method saliva was added to dmLT previously bound to ELISA plates. Plates were tested at the same specified time points.