Sequential MAVS- and MyD88/TRIF-signaling triggers anti-viral responses of tick-borne encephalitis virus-infected murine astrocytes

Upon tick borne encephalitis virus exposure of brain-resident cells, astrocytes are important IFN-β producers that followed a biphasic response, which initially depends on MAVS- and later on MyD88/TRIF-signaling Overall, there are 18 samples of primary astrocytes isolated from two mouse genotypes, C57BL/6 and B6.STOCK-Mavs(tm1Tsc) (MAVS -/-) either PBS treated or infected with tick borne encephalitis virus (TBEV). For each genotype, 3 uninfected, 3 TBEV infected 12 hpi and 3 TBEV infected 24hpi (3= biological replicates). RNA were extracted from the astrocytes cell lysates and sequenced on Illumina NovaSeq-6000 with 50 bp paired-end reads configuration.