Single vesicle fusion experiments illustrating the inhibition of the calcium triggered release by the inhibitory peptide SP9

Synaptic neurotransmitter release is mediated by an orchestra of presynaptic proteins that precisely control and trigger fusion between synaptic vesicles and the neuron terminal at the active zone upon an action potential. Critical to this process are the neuronal SNAREs (Soluble N-ethylmaleimide sensitive factor Attachment protein REceptor), the Ca2+-sensor synaptotagmin, the activator/regulator complexin, and other factors. Here we present the data for single vesicle fusion experiments with synaptic vesicles in absence and presence of the inhibitory peptide SP9 that illustrate the inhibition of calcium-triggered release.Â