SANS Study of interaction of Monoclonal Antibody with Non-ionic Surfactant

Although we have made an extensive study of surface and interfacial adsorption of an engineered monoclonal antibody COE3, its solution structure has never been studied. As we know how COE3 is modified from the native IgG1, the proposed work here will lead to a better understanding of its conformation together with its constituting Fab and Fc in solution under different pHs. Then, we will examine how Fab, Fc and the whole mAb interact with modified Tween 80 with 7 and 13 EOs (PS80(7EO), PS80(13EO)). Preliminary studies have indicated that COE3 would interact with PS80(7EO) more favourably than the ones with longer EOs. Through appropriate contrast variations, SANS will help unravel the structural details relating to the binding of the nonionic surfactants to COE3 or its fragments. We will explore the new SASview based MD simulations for data analysis via support from the NIST team.