CRISPR reagents for Spaulding & Updike, 2024

Intrinsically disordered RG/RGG repeat domains are enriched in multilayered biomolecular condensates such as the nucleolus. C. elegans nucleolar RG/RGG repeats are dispensable for nucleolar accumulation and instead contribute to the organization of proteins into sub-nucleolar compartments. How repeats direct sub-nucleolar compartmentalization is unclear. In this study, we drive expression of full-length RG/RGG repeats in the C. elegans germline to test their ability to localize to nucleoli and organize into nucleolar sub-compartments in vivo. We find that repeats accumulate within germ cell nucleoli but do not enrich in the correct sub-compartment.