Transcriptomes alteration of lentoid body induction on LN511-coated culture

In order to examine the transcriptomes alteration of LN511-based lens epithelial stem cells induction, human embryonic stem cells (hESCs) were induced into lentoid body followed the protocol from Yang et al (FASEB J. 2010), on Matrigel-coated or LN511-coated culture. Each group of cells were collected at day 12 and day 18, during the time when lens epithelial stem cells were generated. The samples were named as Ctrl-d12, Ctrl-d18, LN-511-d12 and LN-511-d18, respectively, (3 replicates, -1/2/3) and sent for RNA-sequence using Illumina HiSeq2500. It turned out that LN511 acted as a robust signaling factor for lens epithelial stem cells induction via enhancing the expression of lens specification related transcription factor, promoting cell proliferation, and suppressing Hippo/Yap signaling. These results provided a valuable resource for studying the mechanisms regulating in vitro lens epithelial stem cells induction. To examine the transcriptomes alteration, each group of cells, on either Matrigel-coated or LN511-coated culture, were collected at day 12 and day 18, respectively. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells at two time points (3 replicates, -1/2/3). Comparative gene expression profiling analysis of RNA-seq for cells on Matrigel-coated culture and that on LN511-coated culture.