Decreased number of antigen-specific CD8⁺ T cells in <i>Btla</i>^−/− mice after LM-OVA infection.

A- WT and Btla−/− mice were orally infected with 1×109 LM-OVA and 7 days p.i., OVA-specific CD8+ T cells were detected by staining spleen cells with OVA-tetramer (Kb/OVA257–264). The percentage and absolute number of OVA-tetramer+ CD8+ T cells found in WT (circles and open bars) and Btla−/− (squares and filled bars) mice are shown. Each symbol represents an individual mouse from a total of 11–14 mice per group, pooled from three independent experiments. B- IFNγ production by CD8+ T cells isolated from WT and Btla−/− mice, 7 days p.i., was calculated by intracellular staining after ex vivo stimulation of the cells with OVA257–264 peptide. The percentage and absolute number of IFNγ+CD8+ T cells is shown. Data shown correspond to the average of 4–6 mice per group and are representative of three independent experiments. C- OVA-tetramer staining in MLN cells and IEL isolated from LM-OVA infected animals, 7 days p.i. Data pooled from three independent experiments are shown. D- Reduced number of memory CD8+ T cells in Btla−/− mice. The percentage and absolute number of OVA-specific memory CD8+ T cells in the spleen of LM-OVA-infected animals was calculated by staining cells with OVA-tetramers, 70 days p.i. E- IFNγ intracellular staining performed on CD8+ T cells isolated from infected mice 70 days p.i., restimulated ex vivo with OVA257–264 peptide. Data shown correspond to the average of 4 mice per group and are representative of three independent experiments.

A- 野生型（WT）与Btla基因敲除（Btla−/−）小鼠经口感染1×10^9个LM-OVA，于感染后7天（p.i., post infection），通过OVA四聚体（OVA-tetramer，Kb/OVA257–264）染色脾脏细胞以检测OVA特异性CD8+ T细胞。本部分展示了WT组（圆形与空白条）与Btla−/−组（方形与填充条）小鼠中OVA-tetramer阳性（OVA-tetramer+）CD8+ T细胞的百分比与绝对计数。每组共11~14只个体小鼠，数据合并自3次独立实验，每个符号代表单只小鼠。
B- 感染后7天，从WT和Btla−/−小鼠中分离的CD8+ T细胞经OVA257–264肽体外刺激（ex vivo stimulation）后，通过细胞内染色检测干扰素γ（IFNγ）的产生水平。本部分展示了IFNγ阳性CD8+ T细胞（IFNγ+CD8+ T）的百分比与绝对计数。该数据为每组4~6只小鼠的平均值，是3次独立实验的代表性结果。
C- 感染后7天，对LM-OVA感染小鼠的肠系膜淋巴结（mesenteric lymph node, MLN）细胞与肠上皮淋巴细胞（intestinal epithelial lymphocyte, IEL）进行OVA四聚体染色，本部分展示合并自3次独立实验的数据。
D- Btla−/−小鼠的记忆性CD8+ T细胞数量减少。感染后70天，通过OVA四聚体染色检测LM-OVA感染小鼠脾脏中OVA特异性记忆性CD8+ T细胞的百分比与绝对计数。
E- 感染后70天，分离感染小鼠的CD8+ T细胞，经OVA257–264肽体外复刺激（ex vivo restimulation）后进行干扰素γ（IFNγ）细胞内染色。该数据为每组4只小鼠的平均值，是3次独立实验的代表性结果。